Investigação da toxicidade genética de produtos naturais através de bioensaios de curta duração
Natural products derived from plants are used as raw material for the synthesis of phytotherapeutics, and are widely used by many populations around the world. Among these compounds, there are the saponins and its metabolites. Its intense use is due to its bioactive properties, added to its struc...
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| Formato: | Dissertação |
| Idioma: | por |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/20332 |
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| Resumo: | Natural products derived from plants are used as raw material for the synthesis of
phytotherapeutics, and are widely used by many populations around the world.
Among these compounds, there are the saponins and its metabolites. Its intense use
is due to its bioactive properties, added to its structural similarity to hormones.
Saponins derived in sapogenins, as Diosgenin and Hecogenin. Besides its use by
pharmaceutical industries to synthesis of steroidal hormones and anti-inflammatories,
these two sapogenins are also used as phytotherapeutics, being also present in
vegetables from diets of many populations. Despite the widespread use of these
compounds, there are a lack of information about their genetic toxicity, which makes
the use of these sapogenins a risk. Given this context, the present work aimed to
investigate the toxic potential of Diosgenin and Hecogenin, at cellular and DNA level.
Assays with HepG2 cells was performed to evaluate the cytotoxicity, genotoxicity and
mutagenicity. Considering the results obtained with Diosgenin, that sapogenin
showed cytotoxic effects at concentrations higher than 30 M, as well as significantly
induced increases in frequency of DNA damage and micronucleus. On the other
hand, Hecogenin only revealed cytotoxicity above 150 M, however, at
concentrations below this value, it was able to induce DNA damage without promote
an increase in micronucleus frequency. The analysis of these results together
suggests that the consumption of foods, phyotheraputics or medicines containing
Diosgenin on its composition, presents a higher risk of toxicity when compared with
formulations with Hecogenin, since Diosgenin shows a mutagenic potential
demonstrated by a significant increase in micronucleus frequency, which are
biomarkers of genome instability. |
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