Live Imaging of Adult Neural Stem Cells in Rodents

The generation of cells of the neural lineage within the brain is not restricted to early development. New neurons, oligodendrocytes, and astrocytes are produced in the adult brain throughout the entire murine life. However, despite the extensive research performed in the field of adult neurogene...

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Principais autores: Ortega, Felipe, Costa, Marcos Romualdo
Formato: article
Idioma:eng
Publicado em: Universidade Federal do Rio Grande do Norte
Assuntos:
live imaging
aNSCs
neurogenic niches
timelapse videomicroscopy
lineage tracing
Endereço do item:https://repositorio.ufrn.br/jspui/handle/123456789/19997
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spelling ri-123456789-199972021-07-09T20:42:23Z Live Imaging of Adult Neural Stem Cells in Rodents Live Imaging of Adult Neural Stem Cells in Rodents Ortega, Felipe Costa, Marcos Romualdo live imaging aNSCs neurogenic niches timelapse videomicroscopy lineage tracing The generation of cells of the neural lineage within the brain is not restricted to early development. New neurons, oligodendrocytes, and astrocytes are produced in the adult brain throughout the entire murine life. However, despite the extensive research performed in the field of adult neurogenesis during the past years, fundamental questions regarding the cell biology of adult neural stem cells (aNSCs) remain to be uncovered. For instance, it is crucial to elucidate whether a single aNSC is capable of differentiating into all three different macroglial cell types in vivo or these distinct progenies constitute entirely separate lineages. Similarly, the cell cycle length, the time and mode of division (symmetric vs. asymmetric) that these cells undergo within their lineage progression are interesting questions under current investigation. In this sense, live imaging constitutes a valuable ally in the search of reliable answers to the previous questions. In spite of the current limitations of technology new approaches are being developed and outstanding amount of knowledge is being piled up providing interesting insights in the behavior of aNSCs. Here, we will review the state of the art of live imaging as well as the alternative models that currently offer new answers to critical questions. The generation of cells of the neural lineage within the brain is not restricted to early development. New neurons, oligodendrocytes, and astrocytes are produced in the adult brain throughout the entire murine life. However, despite the extensive research performed in the field of adult neurogenesis during the past years, fundamental questions regarding the cell biology of adult neural stem cells (aNSCs) remain to be uncovered. For instance, it is crucial to elucidate whether a single aNSC is capable of differentiating into all three different macroglial cell types in vivo or these distinct progenies constitute entirely separate lineages. Similarly, the cell cycle length, the time and mode of division (symmetric vs. asymmetric) that these cells undergo within their lineage progression are interesting questions under current investigation. In this sense, live imaging constitutes a valuable ally in the search of reliable answers to the previous questions. In spite of the current limitations of technology new approaches are being developed and outstanding amount of knowledge is being piled up providing interesting insights in the behavior of aNSCs. Here, we will review the state of the art of live imaging as well as the alternative models that currently offer new answers to critical questions. 2016-03-09T12:04:36Z 2016-03-09T12:04:36Z 2016 article Ortega F and Costa MR (2016) Live Imaging of Adult Neural Stem Cells in Rodents. Front. Neurosci. 10:78. doi: 10.3389/fnins.2016.00078 https://repositorio.ufrn.br/jspui/handle/123456789/19997 eng Acesso Aberto application/pdf Universidade Federal do Rio Grande do Norte Brasil UFRN
institution Repositório Institucional
collection RI - UFRN
language eng
topic live imaging
aNSCs
neurogenic niches
timelapse videomicroscopy
lineage tracing
spellingShingle live imaging
aNSCs
neurogenic niches
timelapse videomicroscopy
lineage tracing
Ortega, Felipe
Costa, Marcos Romualdo
Live Imaging of Adult Neural Stem Cells in Rodents
description The generation of cells of the neural lineage within the brain is not restricted to early development. New neurons, oligodendrocytes, and astrocytes are produced in the adult brain throughout the entire murine life. However, despite the extensive research performed in the field of adult neurogenesis during the past years, fundamental questions regarding the cell biology of adult neural stem cells (aNSCs) remain to be uncovered. For instance, it is crucial to elucidate whether a single aNSC is capable of differentiating into all three different macroglial cell types in vivo or these distinct progenies constitute entirely separate lineages. Similarly, the cell cycle length, the time and mode of division (symmetric vs. asymmetric) that these cells undergo within their lineage progression are interesting questions under current investigation. In this sense, live imaging constitutes a valuable ally in the search of reliable answers to the previous questions. In spite of the current limitations of technology new approaches are being developed and outstanding amount of knowledge is being piled up providing interesting insights in the behavior of aNSCs. Here, we will review the state of the art of live imaging as well as the alternative models that currently offer new answers to critical questions.
format article
author Ortega, Felipe
Costa, Marcos Romualdo
author_facet Ortega, Felipe
Costa, Marcos Romualdo
author_sort Ortega, Felipe
title Live Imaging of Adult Neural Stem Cells in Rodents
title_short Live Imaging of Adult Neural Stem Cells in Rodents
title_full Live Imaging of Adult Neural Stem Cells in Rodents
title_fullStr Live Imaging of Adult Neural Stem Cells in Rodents
title_full_unstemmed Live Imaging of Adult Neural Stem Cells in Rodents
title_sort live imaging of adult neural stem cells in rodents
publisher Universidade Federal do Rio Grande do Norte
publishDate 2016
url https://repositorio.ufrn.br/jspui/handle/123456789/19997
work_keys_str_mv AT ortegafelipe liveimagingofadultneuralstemcellsinrodents
AT costamarcosromualdo liveimagingofadultneuralstemcellsinrodents
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