O antagonismo do receptor de serotonina do tipo 7 da substância cinzenta periaquedutal dorsal reduz a ansiedade experimental basal, mas não aquela gerada pela retirada do etanol em ratos
Ethanol-dependent individuals who reduce or discontinue its use may present Alcohol Withdrawal Syndrome, which is characterized by unpleasant signs and symptoms, such as anxiety, that may trigger relapses. Ethanol, a psychotropic drug, is able to promote behavioral and neurophysiological changes,...
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Formato: | Dissertação |
Idioma: | por |
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Universidade Federal do Rio Grande do Norte
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Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/19862 |
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Resumo: | Ethanol-dependent individuals who reduce or discontinue its use may present
Alcohol Withdrawal Syndrome, which is characterized by unpleasant signs and
symptoms, such as anxiety, that may trigger relapses. Ethanol, a psychotropic drug,
is able to promote behavioral and neurophysiological changes, acting on different
neurotransmitter systems, including the serotonergic, which has also been directly
associated with aversive states, including anxiety. This study aimed to investigate the
participation of type 7 serotonin receptor (5-HT7) of the dorsal periaqueductal gray
(DPAG) on basal experimental anxiety and that caused by ethanol withdrawal. For
this, 75-100 days old Wistar rats were subjected to two experiments. On the first one,
animals underwent stereotactic surgery for implantation of guide cannulas used for
administration of the drug directly into the DPAG. After seven days, the animals
received doses of 2.5; 5 and 10 nmols of type 7 receptor antagonist SB269970 (SB)
or vehicle intra-DPAG and, ten minutes after, they were exposed to elevated plus
maze (EPM). In the following day, the animals were submitted to the same treatment
and tested in the open field (OF). In the second experiment, animals received
increasing concentrations (2%, 4%, 6%) of ethanol as the only source of liquid diet or
water (control group), both with free access to chow. Seventy two hours and ninety
six hours after the ethanol withdrawal, animals received SB (2.5 and 5.0 nmols) intraDPAG
ten minutes before the test in the LCE and OF, respectively. In experiment 1,
the dose of antagonist 10 nmols was able of reversing the anxiety generated by
EPM. In the experiment 2, ineffective SB doses on the LCE (2.5 and 5.0 nmol) were
not able to reverse the anxiety caused by the ethanol withdrawal in the EPM,
although the dose of 2.5 nmols of SB has reversed its hipolocomotor effect in this
test. This result suggests that the 5-HT7 receptor is involved in the modulation of the
basal experimental anxiety in rats, but not in the anxiety caused by ethanol
withdrawal in the DPAG. |
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