Obtenção e conservação de espermatozoides de cutia (Dasyprocta leporina Linneaus, 1753) do semiárido brasileiro
The general objective of this thesis was to stablish protocols to obtain and conserve agouti (Dasyprocta leporina) sperm bred in captivity in the Brazilian semi-arid, aiming its sustainable production. The thesis was divided in three experiments. In the first one, we studied the influence of the...
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Formato: | doctoralThesis |
Idioma: | por |
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Universidade Federal do Rio Grande do Norte
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Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/19811 |
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Resumo: | The general objective of this thesis was to stablish protocols to obtain and conserve
agouti (Dasyprocta leporina) sperm bred in captivity in the Brazilian semi-arid, aiming
its sustainable production. The thesis was divided in three experiments. In the first one,
we studied the influence of the interaction between two probes (quadratics and sine
waves) and two stimulation protocols (continuous and in series) on the agouti sperm
collection by electroejaculation efficiency. The most efficient interaction on this
obtainment was the one with probes with rings associated with stimuli in series (4/7;
57%, P<0.05). In the second experiment we compared the cryoprotectant effects of
different substances (glycerol, ethyleneglycol, dimethylsulfoxide, dimethylformamide)
on epididymis sperm cryopreservation. The highest values on motility (39.5±4.6%),
vigor (2.9±0.2) and membrane integrity (30.6±4.5%) were observed on the samples
cryopreserved using glycerol when compared to those with ethyleneglycol and
dimethylformamide, but there was no difference (P>0.05) when compared to the
samples cryopreserved with dimethylsulfoxide. At last, we studied the effects of the
methods to obtain sperm (electroejaculation vs epididymal collection) on post thawing
sperm quality. The samples obtained by epididymal retrograde flushing showed values
for motility of 25.0±10.9% and vigor 2.4±0.8, and those obtained by electroejaculation
had 31.2±14.2% of motility and vigor of 2.2±0.7, however, without statistical difference
(P>0.05), which shows the possibility to successfully use the epididymal sperm
cryopreservation protocol on agouti ejaculated sperm. In conclusion, significant
advances on obtainment and processing of agouti sperm were made, allowing the
establishment of germplasm banks from sperm samples obtained from the epididymis or
by electroejaculation. |
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