Caracterização de um homólogo de HINT1 em cana-de-açúcar encontrado em bibliotecas subtrativas de CDNA para floração
The sugarcane is a monocot plant grown in tropical and subtropical regions, with Brazil being the largest producer. Despite its economic importance, little is known about the molecular flowering process in sugarcane. This physiological process can promote a loss up to 60% in sugar or bioethanol....
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Formato: | Dissertação |
Idioma: | por |
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Universidade Federal do Rio Grande do Norte
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Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/19790 |
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Resumo: | The sugarcane is a monocot plant grown in tropical and subtropical regions, with
Brazil being the largest producer. Despite its economic importance, little is known
about the molecular flowering process in sugarcane. This physiological process can
promote a loss up to 60% in sugar or bioethanol. Thus, this work had as objective
characterize a HINT1 homologous gene previously identified in subtractive libraries
of flowering. Genomic analysis of gene and promoter region structure allowed the
observation that there are at least two distinct genes homologous to HINT on
sugarcane. Bioinformatics analyses showed the conservation of the characteristic
protein domain of HIT superfamily and indicate a phylogenetic relationship
associated to cell location. Moreover, a possible relation with the SBTILISIN-like
protein family through the information available in interatomas was observed. This
suggests that the HINT gene of sugarcane can be related to plant development,
there are several possibilities of interactions in the regulation of floral induction
process, because the sequences present in regulatory regions indicate that
differential expression of HINT was related to with climatic factors in the Northeast
region of Brazil as well as to biotic stress and phytohormones. Furthermore, the
sugarcane phenotypes indicate that the influence of HINT may happen due to
product accumulation of its enzymatic activity. For these characteristics this gene can
be used as a marker in the selection of new varieties. |
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