Efeito do tratamento com as drogas antipsicóticas haloperidol e clozapina sobre a infecção de toxoplasma gondii em cultura de células retinianas embrionárias
T. gondii is an obligate intracellular protozoan and the main cause of retinochoroiditis in humans. The aim of this study was to evaluate the effect of the antipsychotic drugs haloperidol and clozapine on the course of infection by T. gondii of cultured embryonic retinal cells. Embryo retinas of Gal...
Na minha lista:
| Autor principal: | |
|---|---|
| Outros Autores: | |
| Formato: | Dissertação |
| Idioma: | por |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
|
| Assuntos: | |
| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/17340 |
| Tags: |
Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
|
| Resumo: | T. gondii is an obligate intracellular protozoan and the main cause of retinochoroiditis
in humans. The aim of this study was to evaluate the effect of the antipsychotic drugs
haloperidol and clozapine on the course of infection by T. gondii of cultured
embryonic retinal cells. Embryo retinas of Gallus gallus domesticus (E12) were used
for the preparation of mixed monolayer cultures of retinal cells. Cultures were
maintained on plates of 96 and 24 wells by 37°C in DMEM medium supplemented
with 5% fetal bovine serum for 2 days. After this period, cultures were simultaneously
infected with tachyzoites of T. gondii and treated with the antipsychotics haloperidol
and clozapine for 48 hours. Treatment effects were determined by both assessing
cell viability with the MTT method and evaluating infection outcomes in slides stained
with Giemsa. The treatment with haloperidol and clozapine cells infected with T.
gondii resulted in higher viability of these cells, suggesting a possible prevention of
neuronal degeneration induced by T. gondii. Additionally, intracellular replication of
this protozoan in cells treated with haloperidol and clozapine were significantly
reduced, possibly by modulation of the parasite s intracellular calcium concentration |
|---|