Associação do receptor toll-like 2 com o estado pró-inflamatório do diabetes tipo 1
Inflammation has been pointed out as an important factor in development of chronic diseases, as diabetes. Hyperglycemia condition would be responsible by toll-like receptors, TLR2 and TLR4, and, consequently by local and systemic inflammation induction. Thus, the objective of present study was to...
Na minha lista:
Autor principal: | |
---|---|
Outros Autores: | |
Formato: | Dissertação |
Idioma: | por |
Publicado em: |
Universidade Federal do Rio Grande do Norte
|
Assuntos: | |
Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/13456 |
Tags: |
Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
|
Resumo: | Inflammation has been pointed out as an important factor in development of chronic
diseases, as diabetes. Hyperglycemia condition would be responsible by toll-like
receptors, TLR2 and TLR4, and, consequently by local and systemic inflammation
induction. Thus, the objective of present study was to evaluate type 1 Diabetes mellitus
(T1DM) pro-inflammatory state through mRNA expression of TLRs 2 and 4 and proinflammatory
cytokines IL-1β, IL-6 and TNF-α correlating to diabetic nephropathy. In
order to achieve this objective, 76 T1DM patients and 100 normoglycemic (NG)
subjects aged between 6 and 20 years were evaluated. T1DM subjects were evaluated as
a total group DM1, and considering glycemic control (good glycemic control
DM1G, and poor glycemic control DM1P) and considering time of diagnosis (before
achieving 5 years of diagnosis DM1< 5yrs, and after achieving 5 years of diagnosis
DM1 <5yrs). Metabolic control was evaluated by glucose and glycated hemoglobin
concentrations; to assess renal function serum urea, creatinine, albumin, total protein
and urinary albumin-to-creatinine ratio were determined and to evaluate hepatic
function, AST and ALT serum activities were measured. Pro-inflammatory status was
assessed by mRNA expression of TLRs 2 and 4 and the inflammatory cytokines IL-1β,
IL-6 and TNF-α. Except for DM1G group (18.4%), DM1NC patients (81.6%) showed a
poor glycemic control, with glycated hemoglobin (11,2%) and serum glucose (225,5
md/dL) concentrations significantly increased in relation to NG group (glucose:
76,5mg/dL and glycated hemoglobin: 6,9%). Significantly enhanced values of urea
(20%) and ACR (20,8%) and diminished concentrations of albumin (5,7%) and total
protein (13,6%) were found in T1DM patients, mainly associated to a poor glycemic
control (DM1P increased values of urea: 20% and ACR:49%, and diminished of
albumin: 13,6% and total protein:13,6%) and longer disease duration (DM1 <5yrs -
increased values of urea: 20% and ACR:20,8%, and diminished of albumin: 14,3% and
total protein:13,6%). As regarding pro-inflammatory status evaluation, significantly
increased mRNA expressions were presented for TLR2 (37,5%), IL-1β (43%), IL-6
(44,4%) and TNF-α (15,6%) in T1DM patients in comparison to NG, mainly associated
to DM1P (poor glycemic control TLR2: 82%, IL-1β: 36,8% increase) and DM1 <5yrs
(longer time of diagnosis TLR2: 85,4%, IL-1β: 46,5% increased) groups. Results
support the existence of an inflammatory state mediated by an increased expression of
TLR2 and pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in T1DM |
---|