Bioprospecção de extratos metanólicos de macroalgas marinhas do litoral do Rio Grande do Norte: avaliação das atividades anticoagulante, antioxidante e antiproliferativa
Seaweeds are organisms known to exhibit a variety of biomolecules with pharmacological properties. The coast of Rio Grande do Norte has over 100 species of seaweeds, most of them not yet explored for their pharmacological potential. Sugars and phenolic compounds are the most studied of these bein...
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| Format: | Dissertação |
| Język: | por |
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Universidade Federal do Rio Grande do Norte
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| Hasła przedmiotowe: | |
| Dostęp online: | https://repositorio.ufrn.br/jspui/handle/123456789/13091 |
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| Streszczenie: | Seaweeds are organisms known to exhibit a variety of biomolecules with
pharmacological properties. The coast of Rio Grande do Norte has over 100 species
of seaweeds, most of them not yet explored for their pharmacological potential.
Sugars and phenolic compounds are the most studied of these being assigned a
range of biological properties, such as anticoagulant , antiinflammatory, antitumor
and antioxidant activities. In this work, we obtained methanolic extracts from thirteen
seaweeds of the coast of Rio Grande do Norte (Dictyota cervicornis; Dictiopterys
delicatula; Dictyota menstruallis; D. mertensis; Sargassum filipendula; Spatoglossum
schröederi; Acanthophora specifera; Botryocladia occidentalis; Caulerpa
cupresoides; C. racemosa; C. prolifera; C. sertularioides e Codium isthmocladum).
They were evaluated as anticoagulant and antioxidant drugs, as well as
antiproliferative drugs against the tumor cell line HeLa. None of the methanolic
extracts showed anticoagulant activity, but when they were evaluated as antioxidant
drugs all of extracts showed antioxidant activity in all tests performed (total
antioxidant capacity, sequestration of superoxide and hydroxyl radicals, ferric
chelation and reductase activity), especially the algae D. mentrualis, D. cilliolata and
C. prolifera, who had the greatest potential to donate electrons.In addition, the ability
of iron ions chelation appears as the main antioxidant mechanism of the methanolic
extracts of these seaweeds mainly for the extract of the C. racemosa seaweed, which
reached almost 100% activity. In the MTT assay, all extracts showed inhibitory
activity at different levels againts HeLa cells. Moreover, D. cilliolata (MEDC) and D.
menstrualis (MEDM) extracts showed specific activity to this cell line, not inhibiting
the viability of 3T3 normal cell line, so they were chosen for detailing the
antiproliferative mechanism of action. Using flow cytometry, fluorescence microscopy
and in vitro assays we demonstrated that MEDC and MEDM induced apoptosis in
HeLa cells by activation of caspases 3 and 9 and yet, MEDC induces cell cycle arrest
in S phase. Together, these results showed that the methanolic extracts of brown
seaweed D. menstrualis and D. cilliolata may contain agents with potential use in
combatting cells from human uterine adenocarcinoma. This study also points to the
need for more in-depth research on phytochemical and biological context to enable
the purification of biologically active products of these extracts |
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