Análise de cDNAs identificados em bibliotecas substrativas de cDNA para floração de variedades de cana-de-açúcar cultivadas no Rio Grande do Norte(RN): fator de transição NAC, Calmodulina e Fosfatidiltransferase
The flowering is a physiological process that it is vital for plants. This physiological process has been well studied in the plant model Arabidopsis, but in sugarcane this process is not well known. The transition of the shoot apical meristem from vegetative to flowering is a critical factor for...
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| Formato: | Dissertação |
| Idioma: | por |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/13077 |
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| Resumo: | The flowering is a physiological process that it is vital for plants. This physiological
process has been well studied in the plant model Arabidopsis, but in sugarcane this
process is not well known. The transition of the shoot apical meristem from
vegetative to flowering is a critical factor for plant development. At Brazil northeastern
region, the transition to flowering in sugarcane has an important effect as it may
reduce up to 60% its production. This is a consequence of the sugar translocation
from stalks to the shoot apical meristem which is necessary during the flowering
process. Therefore, the aim of this work was to explore and analyze cDNAs
previously identified using subtractive cDNA libraries. The results showed that these
cDNAs showed differential expression profile in varieties of sugarcane (early x late
flowering). The in silico analysis suggested that these cDNAs had homology to
calmodulin, NAC transcription factor and phosphatidylinositol, a SEC14, which were
described in the literature as having a role in the process of floral development. To
better understand the role of the cDNA homologous to calmodulin, tobacco plants
were transformed with overexpression cassettes in sense and antissense orientation.
Plants overexpressing the cassette in sense orientation did not flowered, while plants
overexpressing the cassette in the antissense orientation produced flowers. The data
obtained in this study suggested the possible role from CAM sequence, SEC14 and
NAC in the induction/floral development pathway in sugarcane, this is the first study
in order to analyze these genes in the sugarcane flowering process. |
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