Propriedades antioxidante, anti-hemostástica e antiproliferativa de galactanas sulfatadas da alga vermelha hypnea musciformis (wulfen) j. V. Lamouroux
Marine algae are one of the major sources of biologic compounds. In extracellular matrix of these organisms there are sulfated polysaccharides that functions as structural components and provides protection against dehydration. The fraction 1.0 (F1.0) rich in sulfated galactans obtained from red...
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| Formato: | Dissertação |
| Idioma: | por |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/12589 |
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| Resumo: | Marine algae are one of the major sources of biologic compounds. In
extracellular matrix of these organisms there are sulfated polysaccharides that
functions as structural components and provides protection against dehydration.
The fraction 1.0 (F1.0) rich in sulfated galactans obtained from red seaweed
Hypnea musciformis was physicochemical characterized and evaluated for
pharmacologic activity through antioxidant activity, cytotoxic action on
erythrocytes, anticoagulant, stimulatory action under antithrombotic heparan
sulfate synthesis and their effects on cell proliferation and cycle cell
progression. The main components of F1.0 were carbohydrates (49.70 ±
0.10%) and sulfate (44.59 ± 0.015%), presenting phenolic compounds (4.79 ±
0.016%) and low protein contamination (0.92 ± 0.001%). Fraction 1.0 showed
polidisperse profile and signs in infrared analysis in 1262, 1074 and 930, 900
and 850 attributed to sulfate esters S=O bond, presence of a 3,6-
anidrogalactose C-O bond, non-sulfated β-D-galactose and a C-O-SO4 bond in
galactose C4, respectively. The fraction rich in sulfated galactans exhibited
strong antioxidant action under lipid peroxidation assay with IC50 of 0.003
mg/mL. Besides the inhibition of hemolysis induced by H2O2 in erythrocytes
treated with F1.0, this fraction did not promote significant cytotoxity under
erythrocytes membranes. F1.0 exhibited low anticoagulant activity causing
moderate direct inhibition of enzimatic activity of thrombin. This fraction
promoted stimulation around of 4.6 times on this synthesis of heparan sulfate
(HS) by rabbit aortic endothelial cells (RAEC) in culture when was compared
with non treated cells. The fraction of this algae displayed antiproliferative action
under RAEC cells causing incresing on cell number on S fase, blocking the
cycle cell progression. Thus F1.0 presented cytostatic and no cytotoxic action
under this cell lineage. These results suggest that F1.0 from H. musciformis
have antioxidant potential which is a great effect for a compound used as food
and in food industry which could be an alternative to food industry to prevent
quality decay of lipid containing food due to lipid peroxidation. These
polysaccharides prevent the lipid peroxidation once the fraction in study
exhibited strong inhibitory action of this process. Furthermore that F1.0 present
strong antithrombotic action promoting the stimulation of antithrombotic HS
synthesis by endothelial cells, being important for thrombosis preventing, by its
inhibitory action under reactive oxygen species (ROS) in some in vitro methods,
being involved in promotion of hypercoagulability state. |
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