Tadalafil and its effects in renal function after kidney ischemia and reperfusion in rats

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Background/purpose: Renal ischemia/reperfusion (I/R) occurs in kidney transplantation and in partial nephrectomyes and it may result in acute tubular necrosis and other postoperative complications. We hypothesized that administration of tadalafil may attenuate circulating inflammatory biomarkers and...

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Principais autores: Carvalho, Ana Luisa Souza Leandro, Araújo, Nina Amanda Borges de, Lima, Raquel Holanda, Menezes, Renata Bezerra, Medeiros, Paulo José de, Medeiros, Aldo Cunha
Formato: Online
Idioma:por
Publicado em: FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
Endereço do item:https://periodicos.ufrn.br/jscr/article/view/8193
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spelling oai:periodicos.ufrn.br:article-81932019-06-05T23:31:07Z Tadalafil and its effects in renal function after kidney ischemia and reperfusion in rats Carvalho, Ana Luisa Souza Leandro Araújo, Nina Amanda Borges de Lima, Raquel Holanda Menezes, Renata Bezerra Medeiros, Paulo José de Medeiros, Aldo Cunha Ischemia Reperfusion Kidney Tadalafil Rats Background/purpose: Renal ischemia/reperfusion (I/R) occurs in kidney transplantation and in partial nephrectomyes and it may result in acute tubular necrosis and other postoperative complications. We hypothesized that administration of tadalafil may attenuate circulating inflammatory biomarkers and improve fasting plasma urea, creatinine and C-reactive protein after renal  ischemia/reperfusion injury in rats. Methods: two groups of Wistar rats we used, both underwent right nephrectomy. Group I/R saline rats (n=6) were subjected to 30 minutes of renal ischemia by clamping the left renal artery and treated with saline; the I/R tadalafil rats (n=6) received 10mg/kg tadalafil by gavage one hour before ischemia. 24 hours after ischemia, laboratory analysis were performed. Results: The anti-inflammatory cytokine IL-10 was increased in the I/R tadalafil (152.8±6.8 pg / dL) rats, compared to I/R saline (53.5±5.2) (p<0.001). TNF-? level was significantly lower in I/R tadalafil group rats compared to I/R saline group (267±11.5 vs. 405.3±12.3), as well as IL-1? (160.4±12.3 vs 274±18.4 pg/dL, respectively; P = 0.0001) Conclusion: We have provided evidence that tadalafil therapy ameliorates circulating pro-inflammatory cytokines in a renal I/R rodent model, while improving kidney function proofs levels and reducing C-reactive protein. Further trials may lead to potential clinical application of tadalafil in cases of renal I/R. FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL 2015-11-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://periodicos.ufrn.br/jscr/article/view/8193 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; Vol. 6 No. 1 (2015); 22-30 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; v. 6 n. 1 (2015); 22-30 2179-7889 por https://periodicos.ufrn.br/jscr/article/view/8193/5903 Copyright (c) 2015 JOURNAL OF SURGICAL AND CLINICAL RESEARCH
institution Periódicos UFRN
collection Portal de Pediódicos Eletrônicos da UFRN
language por
format Online
author Carvalho, Ana Luisa Souza Leandro
Araújo, Nina Amanda Borges de
Lima, Raquel Holanda
Menezes, Renata Bezerra
Medeiros, Paulo José de
Medeiros, Aldo Cunha
spellingShingle Carvalho, Ana Luisa Souza Leandro
Araújo, Nina Amanda Borges de
Lima, Raquel Holanda
Menezes, Renata Bezerra
Medeiros, Paulo José de
Medeiros, Aldo Cunha
Tadalafil and its effects in renal function after kidney ischemia and reperfusion in rats
author_facet Carvalho, Ana Luisa Souza Leandro
Araújo, Nina Amanda Borges de
Lima, Raquel Holanda
Menezes, Renata Bezerra
Medeiros, Paulo José de
Medeiros, Aldo Cunha
author_sort Carvalho, Ana Luisa Souza Leandro
title Tadalafil and its effects in renal function after kidney ischemia and reperfusion in rats
title_short Tadalafil and its effects in renal function after kidney ischemia and reperfusion in rats
title_full Tadalafil and its effects in renal function after kidney ischemia and reperfusion in rats
title_fullStr Tadalafil and its effects in renal function after kidney ischemia and reperfusion in rats
title_full_unstemmed Tadalafil and its effects in renal function after kidney ischemia and reperfusion in rats
title_sort tadalafil and its effects in renal function after kidney ischemia and reperfusion in rats
description Background/purpose: Renal ischemia/reperfusion (I/R) occurs in kidney transplantation and in partial nephrectomyes and it may result in acute tubular necrosis and other postoperative complications. We hypothesized that administration of tadalafil may attenuate circulating inflammatory biomarkers and improve fasting plasma urea, creatinine and C-reactive protein after renal  ischemia/reperfusion injury in rats. Methods: two groups of Wistar rats we used, both underwent right nephrectomy. Group I/R saline rats (n=6) were subjected to 30 minutes of renal ischemia by clamping the left renal artery and treated with saline; the I/R tadalafil rats (n=6) received 10mg/kg tadalafil by gavage one hour before ischemia. 24 hours after ischemia, laboratory analysis were performed. Results: The anti-inflammatory cytokine IL-10 was increased in the I/R tadalafil (152.8±6.8 pg / dL) rats, compared to I/R saline (53.5±5.2) (p<0.001). TNF-? level was significantly lower in I/R tadalafil group rats compared to I/R saline group (267±11.5 vs. 405.3±12.3), as well as IL-1? (160.4±12.3 vs 274±18.4 pg/dL, respectively; P = 0.0001) Conclusion: We have provided evidence that tadalafil therapy ameliorates circulating pro-inflammatory cytokines in a renal I/R rodent model, while improving kidney function proofs levels and reducing C-reactive protein. Further trials may lead to potential clinical application of tadalafil in cases of renal I/R.
publisher FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
publishDate 2015
url https://periodicos.ufrn.br/jscr/article/view/8193
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