Hepatic ischemia-reperfusion and its effects on duodenal anastomosis healing

Background/objetive: The liver ischemia and reperfusion is important for procedures occurring in liver and intestinal trauma injuries that require intestinal resection and anastomosis. The aim of this study was to evaluate the influence of hepatic ischemia/reperfusion in vivo in the healing of duode...

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Principais autores: Oliveira, Caroline Manoela, Oliveira, Catharinne Keyth Mendes de, Almeida, Jéssica Jacinto Salviano de, Medeiros, Vítor Brasil, Nascimento, Rafael Pereira, Azevedo, Italo Medeiros, Medeiros, Aldo Cunha
Formato: Online
Idioma:por
Publicado em: FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
Endereço do item:https://periodicos.ufrn.br/jscr/article/view/8192
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spelling oai:periodicos.ufrn.br:article-81922019-06-05T23:31:07Z Hepatic ischemia-reperfusion and its effects on duodenal anastomosis healing Oliveira, Caroline Manoela Oliveira, Catharinne Keyth Mendes de Almeida, Jéssica Jacinto Salviano de Medeiros, Vítor Brasil Nascimento, Rafael Pereira Azevedo, Italo Medeiros Medeiros, Aldo Cunha Liver Ischemia/reperfusion Duodenum Anastomosis Healing Background/objetive: The liver ischemia and reperfusion is important for procedures occurring in liver and intestinal trauma injuries that require intestinal resection and anastomosis. The aim of this study was to evaluate the influence of hepatic ischemia/reperfusion in vivo in the healing of duodenal anastomoses. Methods: Wistar rats were randomly selected and allocated into two groups of six animals each: liver ischemia/reperfusion + duodenal anastomosis group and duodenal anastomoses group. Hepatic ischemia was induced for 30 minutes by occlusion of the vessels that supply the median and lateral lobes of the liver, using a microvascular clip. In all rats the duodenum was sectioned and an end-to-end anastomosis was performed. After liver reperfusion, animals were observed for 6 days; the maximum bursting pressure of anastomoses was determined and duodenum samples were taken for histopathological examination. Results: As a result, the maximum bursting pressure of anastomosis in ischemia/reperfusion + duodenal anastomosis group (64.5±5.5mmHg) was significantly lower (p<0.001) than in duodenal anastomosis group rats (89.7±3.4mmHg). The inflammatory tissue response in ischemia/reperfusion + duodenal anastomosis group had scores higher than in duodenal anastomosis group rats (p=0.004). Conclusion: In conclusion, the hepatic ischemia-reperfusion negatively affected the healing process of duodenal anastomoses. Key words: Liver. Ischemia/reperfusion. Duodenum. Anastomosis. Healing. FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL 2015-11-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://periodicos.ufrn.br/jscr/article/view/8192 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; Vol. 6 No. 1 (2015); 12-21 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; v. 6 n. 1 (2015); 12-21 2179-7889 por https://periodicos.ufrn.br/jscr/article/view/8192/5902 Copyright (c) 2015 JOURNAL OF SURGICAL AND CLINICAL RESEARCH
institution Periódicos UFRN
collection Portal de Pediódicos Eletrônicos da UFRN
language por
format Online
author Oliveira, Caroline Manoela
Oliveira, Catharinne Keyth Mendes de
Almeida, Jéssica Jacinto Salviano de
Medeiros, Vítor Brasil
Nascimento, Rafael Pereira
Azevedo, Italo Medeiros
Medeiros, Aldo Cunha
spellingShingle Oliveira, Caroline Manoela
Oliveira, Catharinne Keyth Mendes de
Almeida, Jéssica Jacinto Salviano de
Medeiros, Vítor Brasil
Nascimento, Rafael Pereira
Azevedo, Italo Medeiros
Medeiros, Aldo Cunha
Hepatic ischemia-reperfusion and its effects on duodenal anastomosis healing
author_facet Oliveira, Caroline Manoela
Oliveira, Catharinne Keyth Mendes de
Almeida, Jéssica Jacinto Salviano de
Medeiros, Vítor Brasil
Nascimento, Rafael Pereira
Azevedo, Italo Medeiros
Medeiros, Aldo Cunha
author_sort Oliveira, Caroline Manoela
title Hepatic ischemia-reperfusion and its effects on duodenal anastomosis healing
title_short Hepatic ischemia-reperfusion and its effects on duodenal anastomosis healing
title_full Hepatic ischemia-reperfusion and its effects on duodenal anastomosis healing
title_fullStr Hepatic ischemia-reperfusion and its effects on duodenal anastomosis healing
title_full_unstemmed Hepatic ischemia-reperfusion and its effects on duodenal anastomosis healing
title_sort hepatic ischemia-reperfusion and its effects on duodenal anastomosis healing
description Background/objetive: The liver ischemia and reperfusion is important for procedures occurring in liver and intestinal trauma injuries that require intestinal resection and anastomosis. The aim of this study was to evaluate the influence of hepatic ischemia/reperfusion in vivo in the healing of duodenal anastomoses. Methods: Wistar rats were randomly selected and allocated into two groups of six animals each: liver ischemia/reperfusion + duodenal anastomosis group and duodenal anastomoses group. Hepatic ischemia was induced for 30 minutes by occlusion of the vessels that supply the median and lateral lobes of the liver, using a microvascular clip. In all rats the duodenum was sectioned and an end-to-end anastomosis was performed. After liver reperfusion, animals were observed for 6 days; the maximum bursting pressure of anastomoses was determined and duodenum samples were taken for histopathological examination. Results: As a result, the maximum bursting pressure of anastomosis in ischemia/reperfusion + duodenal anastomosis group (64.5±5.5mmHg) was significantly lower (p<0.001) than in duodenal anastomosis group rats (89.7±3.4mmHg). The inflammatory tissue response in ischemia/reperfusion + duodenal anastomosis group had scores higher than in duodenal anastomosis group rats (p=0.004). Conclusion: In conclusion, the hepatic ischemia-reperfusion negatively affected the healing process of duodenal anastomoses. Key words: Liver. Ischemia/reperfusion. Duodenum. Anastomosis. Healing.
publisher FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
publishDate 2015
url https://periodicos.ufrn.br/jscr/article/view/8192
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