The malarial infection can alter the normal biodistribution of a radiopharmaceutical?

Objective: Infection with Plasmodium berghei is a model of murine malaria widely used in experimental studies, similar to Plasmodium falciparum in humans. The aim of this study was to investigate in vivo the influence of P. berghei infection on the biodistribution of sodium pertechnetate (Na99mTcO4)...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Principais autores: Holanda, Cecília Maria de Carvalho Xavier, Barbosa, Vanessa Santos de Arruda, Lisboa, Marcelo José Santiago, Lima, Hilkéa Lima Carla de Sousa Medeiros, Silva, Naisandra Bezerra da, Melo, Luciana Medeiros Bezerra de, Andrade-Neto, Valter Ferreira de, Medeiros, Aldo Cunha
Formato: Online
Idioma:por
Publicado em: FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
Endereço do item:https://periodicos.ufrn.br/jscr/article/view/7268
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id oai:periodicos.ufrn.br:article-7268
record_format ojs
spelling oai:periodicos.ufrn.br:article-72682019-06-05T23:31:09Z The malarial infection can alter the normal biodistribution of a radiopharmaceutical? Holanda, Cecília Maria de Carvalho Xavier Barbosa, Vanessa Santos de Arruda Lisboa, Marcelo José Santiago Lima, Hilkéa Lima Carla de Sousa Medeiros Silva, Naisandra Bezerra da Melo, Luciana Medeiros Bezerra de Andrade-Neto, Valter Ferreira de Medeiros, Aldo Cunha Plasmodium berghei Malaria Bioavailability Radiopharmaceutical 99mTc Pertechnetate Objective: Infection with Plasmodium berghei is a model of murine malaria widely used in experimental studies, similar to Plasmodium falciparum in humans. The aim of this study was to investigate in vivo the influence of P. berghei infection on the biodistribution of sodium pertechnetate (Na99mTcO4) in mice. Methods: 14 Swiss mice were divided into two groups: control (n=7) and treated (n=7). Treated group were inoculated with 1x105 P. berghei infected red blood cells each. On the 15th day, the infected group and the control group received 0.1 mL (0.66 MBq) of Na99mTcO4. After 40 minutes, all animals were killed and kidney, liver, stomach and blood samples were isolated and the percentage of radioactivity per gram of tissue (%ATI/g) was determined. Parts of tissue samples were used for histological analysis. Data were compared by t-Student test and Mann-Whitney test, considering p<0.05 statistically significant. Results: No statistically significant difference in uptake of ATI%/g was observed in any of the organs. Statistically significant histological changes were seen in the liver of the infected group (cell vacuolization and necrosis) and in the kidney (alterations in the parietal cells and mesangial cells). Conclusions: Although the histopathological finding of necrosis has been a significant result, this change did not alter the uptake of technetium-99m at the site of infection. The parasitic infection by acute malaria or the degree of liver injury probably will not affect the performance of nuclear medicine examinations. FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL 2015-06-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://periodicos.ufrn.br/jscr/article/view/7268 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; Vol. 5 No. 2 (2014); 69-79 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; v. 5 n. 2 (2014); 69-79 2179-7889 por https://periodicos.ufrn.br/jscr/article/view/7268/5434 Copyright (c) 2015 JOURNAL OF SURGICAL AND CLINICAL RESEARCH
institution Periódicos UFRN
collection Portal de Pediódicos Eletrônicos da UFRN
language por
format Online
author Holanda, Cecília Maria de Carvalho Xavier
Barbosa, Vanessa Santos de Arruda
Lisboa, Marcelo José Santiago
Lima, Hilkéa Lima Carla de Sousa Medeiros
Silva, Naisandra Bezerra da
Melo, Luciana Medeiros Bezerra de
Andrade-Neto, Valter Ferreira de
Medeiros, Aldo Cunha
spellingShingle Holanda, Cecília Maria de Carvalho Xavier
Barbosa, Vanessa Santos de Arruda
Lisboa, Marcelo José Santiago
Lima, Hilkéa Lima Carla de Sousa Medeiros
Silva, Naisandra Bezerra da
Melo, Luciana Medeiros Bezerra de
Andrade-Neto, Valter Ferreira de
Medeiros, Aldo Cunha
The malarial infection can alter the normal biodistribution of a radiopharmaceutical?
author_facet Holanda, Cecília Maria de Carvalho Xavier
Barbosa, Vanessa Santos de Arruda
Lisboa, Marcelo José Santiago
Lima, Hilkéa Lima Carla de Sousa Medeiros
Silva, Naisandra Bezerra da
Melo, Luciana Medeiros Bezerra de
Andrade-Neto, Valter Ferreira de
Medeiros, Aldo Cunha
author_sort Holanda, Cecília Maria de Carvalho Xavier
title The malarial infection can alter the normal biodistribution of a radiopharmaceutical?
title_short The malarial infection can alter the normal biodistribution of a radiopharmaceutical?
title_full The malarial infection can alter the normal biodistribution of a radiopharmaceutical?
title_fullStr The malarial infection can alter the normal biodistribution of a radiopharmaceutical?
title_full_unstemmed The malarial infection can alter the normal biodistribution of a radiopharmaceutical?
title_sort malarial infection can alter the normal biodistribution of a radiopharmaceutical?
description Objective: Infection with Plasmodium berghei is a model of murine malaria widely used in experimental studies, similar to Plasmodium falciparum in humans. The aim of this study was to investigate in vivo the influence of P. berghei infection on the biodistribution of sodium pertechnetate (Na99mTcO4) in mice. Methods: 14 Swiss mice were divided into two groups: control (n=7) and treated (n=7). Treated group were inoculated with 1x105 P. berghei infected red blood cells each. On the 15th day, the infected group and the control group received 0.1 mL (0.66 MBq) of Na99mTcO4. After 40 minutes, all animals were killed and kidney, liver, stomach and blood samples were isolated and the percentage of radioactivity per gram of tissue (%ATI/g) was determined. Parts of tissue samples were used for histological analysis. Data were compared by t-Student test and Mann-Whitney test, considering p<0.05 statistically significant. Results: No statistically significant difference in uptake of ATI%/g was observed in any of the organs. Statistically significant histological changes were seen in the liver of the infected group (cell vacuolization and necrosis) and in the kidney (alterations in the parietal cells and mesangial cells). Conclusions: Although the histopathological finding of necrosis has been a significant result, this change did not alter the uptake of technetium-99m at the site of infection. The parasitic infection by acute malaria or the degree of liver injury probably will not affect the performance of nuclear medicine examinations.
publisher FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
publishDate 2015
url https://periodicos.ufrn.br/jscr/article/view/7268
work_keys_str_mv AT holandaceciliamariadecarvalhoxavier themalarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT barbosavanessasantosdearruda themalarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT lisboamarcelojosesantiago themalarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT limahilkealimacarladesousamedeiros themalarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT silvanaisandrabezerrada themalarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT melolucianamedeirosbezerrade themalarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT andradenetovalterferreirade themalarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT medeirosaldocunha themalarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT holandaceciliamariadecarvalhoxavier malarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT barbosavanessasantosdearruda malarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT lisboamarcelojosesantiago malarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT limahilkealimacarladesousamedeiros malarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT silvanaisandrabezerrada malarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT melolucianamedeirosbezerrade malarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT andradenetovalterferreirade malarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
AT medeirosaldocunha malarialinfectioncanalterthenormalbiodistributionofaradiopharmaceutical
_version_ 1766682139053522944

Registros relacionados