Effect of Arrabideae chica (crajiru) extract in a model of induced experimental colitis in rats

Objective: This study aimed to investigate the effect of A. chica extract on the evolution of experimental rectocolitis in rats, and the expression of the pro-inflammatory cytokines TNF-a, IL-1? and IL-6 in colonic tissue. Methods: Wistar rats weighing 275±23g were distributed into 4 groups of 6 ani...

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Principais autores: Silva, Evelynne, Medeiros Azevedo, Ítalo, Araújo Filho, Irami, Cunha Medeiros, Aldo
Formato: Online
Idioma:eng
Publicado em: FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
Endereço do item:https://periodicos.ufrn.br/jscr/article/view/27516
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id oai:periodicos.ufrn.br:article-27516
record_format ojs
institution Periódicos UFRN
collection Portal de Pediódicos Eletrônicos da UFRN
language eng
format Online
author Silva, Evelynne
Medeiros Azevedo, Ítalo
Araújo Filho, Irami
Cunha Medeiros, Aldo
spellingShingle Silva, Evelynne
Medeiros Azevedo, Ítalo
Araújo Filho, Irami
Cunha Medeiros, Aldo
Effect of Arrabideae chica (crajiru) extract in a model of induced experimental colitis in rats
author_facet Silva, Evelynne
Medeiros Azevedo, Ítalo
Araújo Filho, Irami
Cunha Medeiros, Aldo
author_sort Silva, Evelynne
title Effect of Arrabideae chica (crajiru) extract in a model of induced experimental colitis in rats
title_short Effect of Arrabideae chica (crajiru) extract in a model of induced experimental colitis in rats
title_full Effect of Arrabideae chica (crajiru) extract in a model of induced experimental colitis in rats
title_fullStr Effect of Arrabideae chica (crajiru) extract in a model of induced experimental colitis in rats
title_full_unstemmed Effect of Arrabideae chica (crajiru) extract in a model of induced experimental colitis in rats
title_sort effect of arrabideae chica (crajiru) extract in a model of induced experimental colitis in rats
description Objective: This study aimed to investigate the effect of A. chica extract on the evolution of experimental rectocolitis in rats, and the expression of the pro-inflammatory cytokines TNF-a, IL-1? and IL-6 in colonic tissue. Methods: Wistar rats weighing 275±23g were distributed into 4 groups of 6 animals each. Rectocolitis was induced in rats by rectal administration of trinitrobenzene sulfonic acid (TNBS). Seventy-two hours after TNBS injection, animals were treated daily for 6 days. Groups: 1. Normal control group without induction of rectocolitis. Received 0.9% saline injection v.o. by gavage during treatment. 2. TNBS rectocolitis group, treated with normal saline (SN) by gavage (TNBS+SN); 3. TNBS rectocolitis group treated with A. chica extract (ACE), receiving a daily dose of 300 mg of A. chica extract by gavage (TNBS+ACE);4. TNBS rectocolitis group treated with mesalazine, receiving a daily dose of 100 mg/kg of mesalazine orally (TNBS+MEZ). Macroscopic examination of the colon and dosing of TNF-?, IL-1? and IL-6 in colon tissue were performed. Results: There was a reduction in weight in animals treated only with TNBS+NS. No difference in weight was observed comparing the animals treated with ACE and MEZ. In the control group no mucosal ulcers or edema of the colon wall were observed. Several mucosal ulcers, edema and hyperemia occurred in the colon of rats in the TNBS+SN group. In two of the animals in this group there was colon perforation, tamponated by omentum. A reduction of mucosal ulcers number in the TNBS+ACE (crajiru) group was seen, compared to the TNBS+SN and TNBS+MEZ group. There was a significant reduction of TNF-?, IL-1? and IL-6 in the colon tissue of animals treated with crajiru extract, TCBS+ACE group, when compared to the control group (p<0.001), TNBS+SN group, and TNBS+MEZ groups (p<0.001). Conclusion: This is the first study to show that A. chica extract positively influences the treatment of TNBS/induced rectocolitis through its antiinflamatory activity. More comprehensive studies are needed to understand the underlying mechanisms.
publisher FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
publishDate 2021
url https://periodicos.ufrn.br/jscr/article/view/27516
work_keys_str_mv AT silvaevelynne effectofarrabideaechicacrajiruextractinamodelofinducedexperimentalcolitisinrats
AT medeirosazevedoitalo effectofarrabideaechicacrajiruextractinamodelofinducedexperimentalcolitisinrats
AT araujofilhoirami effectofarrabideaechicacrajiruextractinamodelofinducedexperimentalcolitisinrats
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spelling oai:periodicos.ufrn.br:article-275162021-12-28T06:07:56Z Effect of Arrabideae chica (crajiru) extract in a model of induced experimental colitis in rats Silva, Evelynne Medeiros Azevedo, Ítalo Araújo Filho, Irami Cunha Medeiros, Aldo Rectocolitis. TNBS. Arrabideae chica. Extract. Treatment. Cytokines. Rats. Objective: This study aimed to investigate the effect of A. chica extract on the evolution of experimental rectocolitis in rats, and the expression of the pro-inflammatory cytokines TNF-a, IL-1? and IL-6 in colonic tissue. Methods: Wistar rats weighing 275±23g were distributed into 4 groups of 6 animals each. Rectocolitis was induced in rats by rectal administration of trinitrobenzene sulfonic acid (TNBS). Seventy-two hours after TNBS injection, animals were treated daily for 6 days. Groups: 1. Normal control group without induction of rectocolitis. Received 0.9% saline injection v.o. by gavage during treatment. 2. TNBS rectocolitis group, treated with normal saline (SN) by gavage (TNBS+SN); 3. TNBS rectocolitis group treated with A. chica extract (ACE), receiving a daily dose of 300 mg of A. chica extract by gavage (TNBS+ACE);4. TNBS rectocolitis group treated with mesalazine, receiving a daily dose of 100 mg/kg of mesalazine orally (TNBS+MEZ). Macroscopic examination of the colon and dosing of TNF-?, IL-1? and IL-6 in colon tissue were performed. Results: There was a reduction in weight in animals treated only with TNBS+NS. No difference in weight was observed comparing the animals treated with ACE and MEZ. In the control group no mucosal ulcers or edema of the colon wall were observed. Several mucosal ulcers, edema and hyperemia occurred in the colon of rats in the TNBS+SN group. In two of the animals in this group there was colon perforation, tamponated by omentum. A reduction of mucosal ulcers number in the TNBS+ACE (crajiru) group was seen, compared to the TNBS+SN and TNBS+MEZ group. There was a significant reduction of TNF-?, IL-1? and IL-6 in the colon tissue of animals treated with crajiru extract, TCBS+ACE group, when compared to the control group (p<0.001), TNBS+SN group, and TNBS+MEZ groups (p<0.001). Conclusion: This is the first study to show that A. chica extract positively influences the treatment of TNBS/induced rectocolitis through its antiinflamatory activity. More comprehensive studies are needed to understand the underlying mechanisms. Objetivo: Investigar o efeito do extrato de Arrabideae chica (crajiru) na evolução da retocolite experimental em ratos, e a expressão das citocinas pró-inflamatórias TNF-a, IL-1? e IL-6 no tecido colônico. Métodos: Ratos Wistar pesando 275±23g foram distribuídos em 4 grupos de 6 animais cada. A retocolite foi induzida nos ratos pela administração retal de ácido trinitrobenzenossulfônico (TNBS). Setenta e duas horas após a injeção de TNBS, os animais foram tratados diariamente durante 6 dias. Grupos: 1. Controle normal sem indução de retocolite. Recebeu injeção de solução salina a 0,9% v.o. por gavagem durante o tratamento. 2. Grupo retocolite com TNBS, tratado com solução salina normal (SN) por gavagem (TNBS + SN); 3. Grupo retocolite com TNBS tratado com extrato de A. chica (ACE), recebendo dose diária de 300 mg de extrato de A. chica por gavagem (TNBS + ACE); 4. Grupo retocolite TNBS tratado com mesalazina, dose diária de 100 mg/kg de mesalazina v.o. (TNBS + MEZ). O exame macroscópico do cólon e a dosagem de TNF-?, IL-1? e IL-6 no tecido do cólon foram realizados. Resultados: Houve redução de peso nos animais tratados apenas com TNBS+NS. Não foram observadas diferenças de peso comparando os animais tratados com ACE e MEZ. No grupo controle não havia úlceras mucosas ou edema da parede do cólon. Várias úlceras da mucosa, edema e hiperemia ocorreram no cólon de ratos no grupo TNBS+SN. Em dois dos animais deste grupo houve perfuração do cólon, tamponada por omento. Foi observada redução das úlceras da mucosa no grupo TNBS+ACE, em comparação com o grupo TNBS + SN e TNBS + MEZ. Houve redução significativa de TNF-?, IL-1? e IL-6 no tecido do cólon de animais tratados com extrato de crajiru, (grupo TCBS+ECA), quando comparados ao grupo controle (p <0,001), e grupos TNBS+SN, e TNBS+MEZ (p <0,001). Conclusão: Este é o primeiro estudo a mostrar que o extrato de A. chica influencia positivamente o tratamento de / retocolite induzida por TNBS, por meio de sua atividade antiinflamatória. Estudos mais abrangentes são necessários para compreender os mecanismos subjacentes. FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL 2021-12-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://periodicos.ufrn.br/jscr/article/view/27516 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; Vol. 12 No. 2 (2021); 68-76 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; v. 12 n. 2 (2021); 68-76 2179-7889 eng https://periodicos.ufrn.br/jscr/article/view/27516/15229 Copyright (c) 2021 JOURNAL OF SURGICAL AND CLINICAL RESEARCH

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