Antioxidant effect of Arrabideae chica (crajiru) extract on oxidative stress in diabetic rats
Objective: This study aimed to investigate the effects of the antioxidant potential of Arrabideae chica (crajiru) extract on oxidative stress in diabetic rats. Methods: Adult Wistar rats (Rattus norvegicus), weighing 238±12g were divided into three groups of six rats each: CN normal untreated contro...
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FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
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Perlles Gomes Bandeira e Sousa, Paula Medeiros Azevedo, Ítalo Cunha Medeiros , Aldo |
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Perlles Gomes Bandeira e Sousa, Paula Medeiros Azevedo, Ítalo Cunha Medeiros , Aldo Antioxidant effect of Arrabideae chica (crajiru) extract on oxidative stress in diabetic rats |
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Perlles Gomes Bandeira e Sousa, Paula Medeiros Azevedo, Ítalo Cunha Medeiros , Aldo |
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Perlles Gomes Bandeira e Sousa, Paula |
| title |
Antioxidant effect of Arrabideae chica (crajiru) extract on oxidative stress in diabetic rats |
| title_short |
Antioxidant effect of Arrabideae chica (crajiru) extract on oxidative stress in diabetic rats |
| title_full |
Antioxidant effect of Arrabideae chica (crajiru) extract on oxidative stress in diabetic rats |
| title_fullStr |
Antioxidant effect of Arrabideae chica (crajiru) extract on oxidative stress in diabetic rats |
| title_full_unstemmed |
Antioxidant effect of Arrabideae chica (crajiru) extract on oxidative stress in diabetic rats |
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antioxidant effect of arrabideae chica (crajiru) extract on oxidative stress in diabetic rats |
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Objective: This study aimed to investigate the effects of the antioxidant potential of Arrabideae chica (crajiru) extract on oxidative stress in diabetic rats. Methods: Adult Wistar rats (Rattus norvegicus), weighing 238±12g were divided into three groups of six rats each: CN normal untreated control; DIAB+NS diabetic rats treated with normal saline; and diabetic rats treated with crajiru extract, DIAB+CR. The CN and DIAB+NS groups (control groups) received normal saline solution (NS) orally (gavage); rats in the DIAB+CR group received crajiru extract (300 mg/kg) once a day by gavage for 6 weeks. Measurements of urea and creatinine in serum, and kidney tissue catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were performed. The variables were assessed using the Tukey test, significance p<0.05. Results: All animals survived the experiments. In the CN group, compared with the DIAB+NS group, there was significant difference between the levels of glycemia on the second day of dosing and on the 10th day (p<0.05). No difference was observed on glycemia comparing the 2th and 10th day on the rats of group C+NS (p>0.05). Diabetic animals from DIAB+CR group had a significant reduction in glycemia on 10th day of treatment, comparing the 2nd day (p<0.05). There was a significant reduction in glycemia in the DIAB+CR group, comparing with the DIAB+NS group (p<0.05). There was an increase in urea and creatinine levels in rats DIAB+SN when compared to controls, C+SN (p<0.001). Rats from the DIAB+CR group had a significant reduction in urea and creatinine, compared to the DIAB+NS group (p<0.001). There were no significant differences in urea and creatinine comparing the C+NS and DIAB+CR groups. The rats from the DIAB+NS group had significantly lower levels of CAT, GSH-px and SOD when compared to the normal control rats (p<0.001). In animals from the DIAB+SN group, the levels of these antioxidant enzymes were significantly reduced (p<0.001). The treatment of diabetics with crajiru extract caused a significant increase (p<0.001) in the levels of CAT, GSH-px and SOD, when compared to rats in the BIAB+SN group. Conclusion: The data of the present study confirms that the crajiru extract positively influenced the control of hyperglycemia in diabetic rats. More research is needed to provide a better understanding of the mechanisms of diabetes treatment using crajiru extract and its flavonoids. |
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FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL |
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2021 |
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https://periodicos.ufrn.br/jscr/article/view/27173 |
| work_keys_str_mv |
AT perllesgomesbandeiraesousapaula antioxidanteffectofarrabideaechicacrajiruextractonoxidativestressindiabeticrats AT medeirosazevedoitalo antioxidanteffectofarrabideaechicacrajiruextractonoxidativestressindiabeticrats AT cunhamedeirosaldo antioxidanteffectofarrabideaechicacrajiruextractonoxidativestressindiabeticrats AT perllesgomesbandeiraesousapaula efeitoantioxidantedoextratodearrabideaechicacrajirunoestresseoxidativoemratosdiabeticos AT medeirosazevedoitalo efeitoantioxidantedoextratodearrabideaechicacrajirunoestresseoxidativoemratosdiabeticos AT cunhamedeirosaldo efeitoantioxidantedoextratodearrabideaechicacrajirunoestresseoxidativoemratosdiabeticos |
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oai:periodicos.ufrn.br:article-271732021-12-27T20:52:38Z Antioxidant effect of Arrabideae chica (crajiru) extract on oxidative stress in diabetic rats Efeito antioxidante do extrato de Arrabideae chica (crajiru) no estresse oxidativo em ratos diabéticos Perlles Gomes Bandeira e Sousa, Paula Medeiros Azevedo, Ítalo Cunha Medeiros , Aldo Diabetes; Rats; Arrabideae chica; Extract; Oxidative stress; Treatment. Objective: This study aimed to investigate the effects of the antioxidant potential of Arrabideae chica (crajiru) extract on oxidative stress in diabetic rats. Methods: Adult Wistar rats (Rattus norvegicus), weighing 238±12g were divided into three groups of six rats each: CN normal untreated control; DIAB+NS diabetic rats treated with normal saline; and diabetic rats treated with crajiru extract, DIAB+CR. The CN and DIAB+NS groups (control groups) received normal saline solution (NS) orally (gavage); rats in the DIAB+CR group received crajiru extract (300 mg/kg) once a day by gavage for 6 weeks. Measurements of urea and creatinine in serum, and kidney tissue catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were performed. The variables were assessed using the Tukey test, significance p<0.05. Results: All animals survived the experiments. In the CN group, compared with the DIAB+NS group, there was significant difference between the levels of glycemia on the second day of dosing and on the 10th day (p<0.05). No difference was observed on glycemia comparing the 2th and 10th day on the rats of group C+NS (p>0.05). Diabetic animals from DIAB+CR group had a significant reduction in glycemia on 10th day of treatment, comparing the 2nd day (p<0.05). There was a significant reduction in glycemia in the DIAB+CR group, comparing with the DIAB+NS group (p<0.05). There was an increase in urea and creatinine levels in rats DIAB+SN when compared to controls, C+SN (p<0.001). Rats from the DIAB+CR group had a significant reduction in urea and creatinine, compared to the DIAB+NS group (p<0.001). There were no significant differences in urea and creatinine comparing the C+NS and DIAB+CR groups. The rats from the DIAB+NS group had significantly lower levels of CAT, GSH-px and SOD when compared to the normal control rats (p<0.001). In animals from the DIAB+SN group, the levels of these antioxidant enzymes were significantly reduced (p<0.001). The treatment of diabetics with crajiru extract caused a significant increase (p<0.001) in the levels of CAT, GSH-px and SOD, when compared to rats in the BIAB+SN group. Conclusion: The data of the present study confirms that the crajiru extract positively influenced the control of hyperglycemia in diabetic rats. More research is needed to provide a better understanding of the mechanisms of diabetes treatment using crajiru extract and its flavonoids. Objetivo: Este estudo teve como objetivo investigar os efeitos do potencial antioxidante do extrato de Arrabideae chica (crajiru) sobre o estresse oxidativo em ratos diabéticos. Métodos: Ratos Wistar adultos (Rattus norvegicus), pesando 238±12g, foram divididos em três grupos de seis ratos cada. Grupo CN controle normal não tratado; Ratos diabéticos tratados com salina normal (DIAB+SN); e ratos diabéticos tratados com extrato de crajiru (DIAB+CR). Diabetes induzida com estreptozotocina. Salina e extrato de crajiru foram injetados por via oral (gavagem); o extrato de crajiru (300 mg/kg) foi usado uma vez ao dia por gavagem por 6 semanas. Foram realizadas medições de uréia e creatinina séricas, e as enzimas antioxidantes catalase (CAT), superóxido dismutase (SOD) e glutationa peroxidase (GPx) no tecido renal dos animais. As variáveis ??foram avaliadas pelo teste de Tukey, significância p<0,05. Resultados: Todos os animais sobreviveram aos experimentos. A glicemia foi significativamente mais baixa no grupo CN, em comparação com o grupo DIAB+NS, tanto no segundo quanto no 10º dia pós indução do diabetes (p<0,05). Não foi observada diferença na glicemia comparando o 2º e o 10º dia nos ratos do grupo C+NS (p>0,05). Animais diabéticos tratados com extrato de crajiru (DIAB+CR), tiveram redução significativa da glicemia, comparando o 2º dia com o 10º dia de tratamento (p<0,05). Houve redução significativa da glicemia no grupo tratado com crajiru (DIAB+CR), comparando-se o grupo DIAB+NS (p<0,05). Houve aumento dos níveis de uréia e creatinina nos ratos DIAB+SN quando comparados aos controles, C+SN (p<0,001). Ratos diabéticos do grupo DIAB+CR apresentaram redução significativa da uréia e creatinina, em comparação ao grupo DIAB+NS (p<0,001). Não houve diferenças significativas na uréia e creatinina entre os grupos C+NS e DIAB+CR. Os ratos do grupo DIAB+NS apresentaram níveis significativamente mais baixos de CAT, GSH-px e SOD quando comparados aos ratos controle normais - CN (p<0,001). O tratamento de diabéticos com extrato de crajiru causou aumento significativo (p<0,001) nos níveis de CAT, GSH-px e SOD, quando comparados aos ratos do grupo BIAB+SN. Conclusão: Os dados do presente estudo confirmam que a o extrato de crajiru influenciou positivamente no controle da hiperglicemia em ratos diabéticos. Mais pesquisas são necessárias para fornecer uma melhor compreensão dos mecanismos de tratamento do diabetes usando extrato de crajiru e seus flavonóides. FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL 2021-12-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://periodicos.ufrn.br/jscr/article/view/27173 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; Vol. 12 No. 1 (2021); 1-12 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; v. 12 n. 1 (2021); 1-12 2179-7889 eng https://periodicos.ufrn.br/jscr/article/view/27173/15208 Copyright (c) 2021 JOURNAL OF SURGICAL AND CLINICAL RESEARCH |