TREATMENT OF ABDOMINAL SEPSIS WITH LEVAMISOLE AND PERITONEAL LAVAGE IN RATS

Introduction: Intra-abdominal sepsis is one of the most difficult situations in surgery and usually presents as a peritonitis. It is a systemic inflammatory response to infection that is often associated with hypoperfusion followed by tissue injury and organ failure. Given that its the sequelae refl...

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Principais autores: Vieira da Silva, Gabriela, Medeiros de Cunha, Aldo, Medeiros de Azevedo, Ítalo, Medeiros Soares Celani, Lívia, Fernandes Santos de Oliveira , Filipe
Formato: Online
Idioma:eng
Publicado em: FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
Endereço do item:https://periodicos.ufrn.br/jscr/article/view/22173
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spelling oai:periodicos.ufrn.br:article-221732020-12-19T17:35:21Z TREATMENT OF ABDOMINAL SEPSIS WITH LEVAMISOLE AND PERITONEAL LAVAGE IN RATS Vieira da Silva, Gabriela Medeiros de Cunha, Aldo Medeiros de Azevedo, Ítalo Medeiros Soares Celani, Lívia Fernandes Santos de Oliveira , Filipe Abdominal sepsis. Peritoneal lavage. Levamisole. Treatment. Rats. Sepse abdominal. Lavagem peritoneal. Levamisol. Tratamento. Ratos. Introduction: Intra-abdominal sepsis is one of the most difficult situations in surgery and usually presents as a peritonitis. It is a systemic inflammatory response to infection that is often associated with hypoperfusion followed by tissue injury and organ failure. Given that its the sequelae reflect the increasing severity of the systemic response to infection, a correct and early treatment needs to be established. In this sense, the study analyzes the effect of peritoneal lavage and the use of the isomers immunomodulator in the treatment of abdominal sepsis in rats. Methods: The animals were randomly assigned to three groups A (control), B (peritoneal lavage), C (levamisole). All groups underwent laparotomy, anesthesia and cecal ligation/puncture. A ten-parameter clinical score was analyzed, which allowed the classification of sepsis degree, peritoneal fluid cultures, and cytokine dosage. Results: Group C had better scores on the clinical score and also lower growth of bacterial colonies in cultures. A lower dosage of TNF-alpha, IL-Ib, IL-6 and PCR in group C was observed, compared to groups A and B. Conclusion: Levamisole positively influenced the treatment in the parameters analyzed compared to the peritoneal lavage group and the control group. Introdução: A sepse intra-abdominal é uma das situações mais difíceis na cirurgia e geralmente se apresenta como peritonite. Trata-se de uma resposta inflamatória sistêmica à infecção que frequentemente associada à hipoperfusão seguida de lesão tecidual e falência orgânica. Tendo em vista que as suas sequelas refletem a gravidade crescente da resposta sistêmica à infecção, é necessário estabelecer um tratamento correto e precoce. Nesse sentido, o estudo analisa o efeito da lavagem peritoneal e o uso do imunomodulador levamisol no tratamento da sepse abdominal em ratos. Métodos: Os animais foram distribuídos randomicamente em três grupos A (controle), B (lavagem peritoneal), C (levamisol). Todos os grupos foram submetidos à laparotomia, anestesia e ligadura/punção cecal. Foi analisado um escore clínico com dez parâmetros o qual permitiu classificar o grau de sepse, além de culturas do líquido peritoneal e dosagem de citocinas. Resultados: O grupo C obteve melhor pontuação no escore clínico e também menor crescimento de colônias bactérias nas culturas do que os grupos A e B. Dosagens significativamente menores das citocinas TNF-alfa, IL-Ib, IL-6 e PCR foram obsdervadas nos ratos do grupo C em comparação com os grupos A e B, respectivamente. Conclusão: O levamisol influiu positivamente no tratamento nos parâmetros analisados em comparação com o grupo da lavagem peritoneal e o controle. FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL 2020-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://periodicos.ufrn.br/jscr/article/view/22173 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; Vol. 11 No. 2 (2020); 73-80 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; v. 11 n. 2 (2020); 73-80 2179-7889 eng https://periodicos.ufrn.br/jscr/article/view/22173/13556 Copyright (c) 2020 JOURNAL OF SURGICAL AND CLINICAL RESEARCH
institution Periódicos UFRN
collection Portal de Pediódicos Eletrônicos da UFRN
language eng
format Online
author Vieira da Silva, Gabriela
Medeiros de Cunha, Aldo
Medeiros de Azevedo, Ítalo
Medeiros Soares Celani, Lívia
Fernandes Santos de Oliveira , Filipe
spellingShingle Vieira da Silva, Gabriela
Medeiros de Cunha, Aldo
Medeiros de Azevedo, Ítalo
Medeiros Soares Celani, Lívia
Fernandes Santos de Oliveira , Filipe
TREATMENT OF ABDOMINAL SEPSIS WITH LEVAMISOLE AND PERITONEAL LAVAGE IN RATS
author_facet Vieira da Silva, Gabriela
Medeiros de Cunha, Aldo
Medeiros de Azevedo, Ítalo
Medeiros Soares Celani, Lívia
Fernandes Santos de Oliveira , Filipe
author_sort Vieira da Silva, Gabriela
title TREATMENT OF ABDOMINAL SEPSIS WITH LEVAMISOLE AND PERITONEAL LAVAGE IN RATS
title_short TREATMENT OF ABDOMINAL SEPSIS WITH LEVAMISOLE AND PERITONEAL LAVAGE IN RATS
title_full TREATMENT OF ABDOMINAL SEPSIS WITH LEVAMISOLE AND PERITONEAL LAVAGE IN RATS
title_fullStr TREATMENT OF ABDOMINAL SEPSIS WITH LEVAMISOLE AND PERITONEAL LAVAGE IN RATS
title_full_unstemmed TREATMENT OF ABDOMINAL SEPSIS WITH LEVAMISOLE AND PERITONEAL LAVAGE IN RATS
title_sort treatment of abdominal sepsis with levamisole and peritoneal lavage in rats
description Introduction: Intra-abdominal sepsis is one of the most difficult situations in surgery and usually presents as a peritonitis. It is a systemic inflammatory response to infection that is often associated with hypoperfusion followed by tissue injury and organ failure. Given that its the sequelae reflect the increasing severity of the systemic response to infection, a correct and early treatment needs to be established. In this sense, the study analyzes the effect of peritoneal lavage and the use of the isomers immunomodulator in the treatment of abdominal sepsis in rats. Methods: The animals were randomly assigned to three groups A (control), B (peritoneal lavage), C (levamisole). All groups underwent laparotomy, anesthesia and cecal ligation/puncture. A ten-parameter clinical score was analyzed, which allowed the classification of sepsis degree, peritoneal fluid cultures, and cytokine dosage. Results: Group C had better scores on the clinical score and also lower growth of bacterial colonies in cultures. A lower dosage of TNF-alpha, IL-Ib, IL-6 and PCR in group C was observed, compared to groups A and B. Conclusion: Levamisole positively influenced the treatment in the parameters analyzed compared to the peritoneal lavage group and the control group.
publisher FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
publishDate 2020
url https://periodicos.ufrn.br/jscr/article/view/22173
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