Effect of hyaluronic acid on skin healing in diabetic rats

Purpose: The objective of this study was to examine the effect of topical hyaluronic acid (HA) treatment on wound healing in diabetic rats. Methods: Wistar rats weighing 279 ± 32g were randomly selected and allocated into 2 groups, with six rats each, all submitted to diabetes induction with strepto...

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Autor principal: MEDEIROS SOARES CELANI, LIVIA
Formato: Online
Idioma:eng
Publicado em: FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
Endereço do item:https://periodicos.ufrn.br/jscr/article/view/18825
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id oai:periodicos.ufrn.br:article-18825
record_format ojs
institution Periódicos UFRN
collection Portal de Pediódicos Eletrônicos da UFRN
language eng
format Online
author MEDEIROS SOARES CELANI, LIVIA
spellingShingle MEDEIROS SOARES CELANI, LIVIA
Effect of hyaluronic acid on skin healing in diabetic rats
author_facet MEDEIROS SOARES CELANI, LIVIA
author_sort MEDEIROS SOARES CELANI, LIVIA
title Effect of hyaluronic acid on skin healing in diabetic rats
title_short Effect of hyaluronic acid on skin healing in diabetic rats
title_full Effect of hyaluronic acid on skin healing in diabetic rats
title_fullStr Effect of hyaluronic acid on skin healing in diabetic rats
title_full_unstemmed Effect of hyaluronic acid on skin healing in diabetic rats
title_sort effect of hyaluronic acid on skin healing in diabetic rats
description Purpose: The objective of this study was to examine the effect of topical hyaluronic acid (HA) treatment on wound healing in diabetic rats. Methods: Wistar rats weighing 279 ± 32g were randomly selected and allocated into 2 groups, with six rats each, all submitted to diabetes induction with streptozotocin. Under anesthesia, a 1 cm2 open wound was made on the back skin of all animals. Group 1 - rats treated with topical 0.9% saline use on open skin wounds. Group 2 - rats treated with topical use of 0.4% HA over skin lesions. After anesthesia on the 10th postoperative day, a biopsy of the skin dorsal wound area, including the entire healing wound, was performed for histopathological examination. Quantitative analysis was performed for the amount of macrophages, vascular neoformation, fibroblasts and collagen fibers, using a digitizer and image analyzer system. Results: All animals included in the study had blood glucose above 250mg/dL on the second day after streptozotocin administration and remained with high blood glucose until the 10th day, when the study was completed. After quantitative evaluation of collagen fibers, fibroblasts, vascular neoformation and macrophages performed in the ImagePro Plus 6.0 software, it was observed that the wound scores of diabetic animals treated with hyaluronic acid (185 ± 15.7) were significantly higher than those treated with saline (134 ± 11.7). The difference was statistically significant (p <0.05). Conclusion: Treatment of open wounds with HA effectively improved the healing process in hyperglycemic rats through an anti-inflammatory effect. This study suggests and provided evidence that topical use of HA is promising in the treatment of wounds in diabetic rats.
publisher FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL
publishDate 2019
url https://periodicos.ufrn.br/jscr/article/view/18825
work_keys_str_mv AT medeirossoarescelanilivia effectofhyaluronicacidonskinhealingindiabeticrats
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spelling oai:periodicos.ufrn.br:article-188252019-10-11T19:55:55Z Effect of hyaluronic acid on skin healing in diabetic rats MEDEIROS SOARES CELANI, LIVIA Wound healing. Diabetes. Hyaluronic acid. Treatment. Animal model. Rats. Purpose: The objective of this study was to examine the effect of topical hyaluronic acid (HA) treatment on wound healing in diabetic rats. Methods: Wistar rats weighing 279 ± 32g were randomly selected and allocated into 2 groups, with six rats each, all submitted to diabetes induction with streptozotocin. Under anesthesia, a 1 cm2 open wound was made on the back skin of all animals. Group 1 - rats treated with topical 0.9% saline use on open skin wounds. Group 2 - rats treated with topical use of 0.4% HA over skin lesions. After anesthesia on the 10th postoperative day, a biopsy of the skin dorsal wound area, including the entire healing wound, was performed for histopathological examination. Quantitative analysis was performed for the amount of macrophages, vascular neoformation, fibroblasts and collagen fibers, using a digitizer and image analyzer system. Results: All animals included in the study had blood glucose above 250mg/dL on the second day after streptozotocin administration and remained with high blood glucose until the 10th day, when the study was completed. After quantitative evaluation of collagen fibers, fibroblasts, vascular neoformation and macrophages performed in the ImagePro Plus 6.0 software, it was observed that the wound scores of diabetic animals treated with hyaluronic acid (185 ± 15.7) were significantly higher than those treated with saline (134 ± 11.7). The difference was statistically significant (p <0.05). Conclusion: Treatment of open wounds with HA effectively improved the healing process in hyperglycemic rats through an anti-inflammatory effect. This study suggests and provided evidence that topical use of HA is promising in the treatment of wounds in diabetic rats. Objetivo: O objetivo deste estudo foi examinar o efeito do tratamento tópico com ácido hialurônico (AH) na cicatrização de feridas em ratos diabéticos. Métodos: Ratos Wistar pesando 279 ± 32g foram selecionados aleatoriamente e alocados em 2 grupos, com seis ratos cada, todos submetidos à indução de diabetes com estreptozotocina. Sob anestesia, foi feita uma ferida aberta de 1 cm2 na pele dorsal de todos os animais. Grupo 1 - ratos tratados com soro fisiológico a 0,9% em feridas abertas na pele. Grupo 2 - ratos tratados com uso tópico de HA de 0,4% sobre lesões cutâneas. Após anestesia no 10º dia de pós-operatório, foi realizada biópsia da área dorsal da pele, incluindo toda a ferida cicatrizante, para exame histopatológico. A análise quantitativa foi realizada para a quantidade de macrófagos, neoformação vascular, fibroblastos e fibras de colágeno, utilizando um sistema de digitalizador e analisador de imagens. Resultados: Todos os animais incluídos no estudo apresentaram glicemia acima de 250 mg / dL no segundo dia após a administração de estreptozotocina e permaneceram com glicemia alta até o décimo dia, quando o estudo foi concluído. Após avaliação quantitativa das fibras colágenas, fibroblastos, neoformação vascular e macrófagos realizados no software ImagePro Plus 6.0, observou-se que os escores de feridas em animais diabéticos tratados com ácido hialurônico (185 ± 15,7) foram significativamente maiores do que os tratados com solução salina (134 ± 11,7). A diferença foi estatisticamente significante (p <0,05). Conclusão: O tratamento de feridas abertas com AH melhorou efetivamente o processo de cicatrização em ratos hiperglicêmicos através de um efeito anti-inflamatório. Este estudo sugere e fornece evidências de que o uso tópico de HA é promissor no tratamento de feridas em ratos diabéticos. FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE, BRAZIL 2019-10-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://periodicos.ufrn.br/jscr/article/view/18825 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; Vol. 10 No. 2 (2019); 76-87 JOURNAL OF SURGICAL AND CLINICAL RESEARCH; v. 10 n. 2 (2019); 76-87 2179-7889 eng https://periodicos.ufrn.br/jscr/article/view/18825/12144 Copyright (c) 2019 JOURNAL OF SURGICAL AND CLINICAL RESEARCH

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