Inferências carioevolutivas sobre grupos crípticos de peixes marinhos e estuarinos/

Mostrar outras versões (1)

Resumo: The genome of all organisms is subject to injuries that can be caused by endogenous and environmental factors. If these lesions are not corrected, it can be fixed generating a mutation which can be lethal to the organisms. In order to prevent this, there are different DNA repair mechanisms....

ver descrição completa

Na minha lista:

Detalhes bibliográficos
Principais autores:	Araújo, Washington Candeia de., Molina, Wagner Franco.
Formato:	Dissertação
Publicado em:
Assuntos:	Citogenética - Dissertação. Evolução cromossômica - Espécies crípticas - Peixes marinhos - Cytogenetic. Karyotipic evolution. Cryptic species. Marine fish.
Endereço do item:	https://repositorio.ufrn.br/bitstream/123456789/16766/1/WashingtonCA.pdf
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!

id	oai:localhost:123456789-83851
record_format	dspace
spelling	oai:localhost:123456789-838512022-11-30T10:57:20Z Inferências carioevolutivas sobre grupos crípticos de peixes marinhos e estuarinos/ Araújo, Washington Candeia de. Molina, Wagner Franco. Citogenética - Dissertação. Evolução cromossômica - Dissertação. Espécies crípticas - Dissertação. Peixes marinhos - Dissertação. Cytogenetic. Karyotipic evolution. Cryptic species. Marine fish. Resumo: The genome of all organisms is subject to injuries that can be caused by endogenous and environmental factors. If these lesions are not corrected, it can be fixed generating a mutation which can be lethal to the organisms. In order to prevent this, there are different DNA repair mechanisms. These mechanisms are well known in bacteria, yeast, human, but not in plants. Two plant models Oriza sativa and Arabidopsis thaliana had the genome sequenced and due to this some DNA repair genes have been characterized. The aim of this work is to characterized two sugarcane cDNAs that had homology to AP endonuclease: scARP1 and scARP3. In silico has been done with these two sequences and other from plants. It has been observed domain conservation on these sequences, but the cystein at 65 position that is a characteristic from the redox domain in APE1 protein was not so conservated in plants. Phylogenetic relationship showed two branches, one branch with dicots and monocots sequence and the other branch with only monocots sequences. Another approach in order to characterized these two cDNAs was to construct overexpression cassettes (sense and antisense orientation) using the 35S promoter. After that, these cassettes were transferred to the binary vector pPZP211. Furthermore, previously in the laboratory was obtained a plant from icotiana tabacum containing the overexpression cassette in anti-sense orientation. It has been observed that this plant had a slow development and problems in setting seeds. After some manual crossing, some seeds were obtained (T2) and it was analyzed the T2 segregation. The third approach used in this work was to clone the promoter region from these two cDNAs by PCR walking. The sequences obtained were analyzed using the program PLANTCARE. It was observed in these sequences some motives that may be related to oxidative stress response.#$&Abstract:Cytogenetic studies have been revealing a great diversity not detected, until then, in several families of fishes. Many of these groups, especially those that exhibit great diversity, like Perciformes and Siluriformes, possess species with difficult morphologic characterization, called cryptic species, commonly detected through karyotypic analyses, which reveals outstanding interespecific variations with relationship to the number and its chromosomal structures. Thus, the present work intends to contribute for the cytogenetic knowledge of marine and brackish fish species, because they peculiar life habits and by lack of cytogenetic data of your genetic aspects. Therefore, cytogenetic studies were developed in a species of Apogonidae (Perciformes), two species of sea catfishes of the family Ariidae (Siluriformes) and brackish fish Paurachenipterus galeatus (Siluriformes, Auchenipteridae), through C banding, Ag-NOR, use of base-specific flourochromes (DAPI and CMA3), as well as FISH (Fluorescent in situ hybridization) using ribosomal DNA probes 5S and 18S. The present results contribute to a better understanding of the processes of differentiation patterns and chromosome evolution in these groups. The use of other approaches (the morphology and molecular tools) will allow a larger understanding of the genetic and biological diversity of the Brazilian ichthyofauna. 1 2022-10-05T20:07:52Z 2022-10-05T20:07:52Z 2009. Dissertação 575 A663i DISSERT 118336 https://repositorio.ufrn.br/bitstream/123456789/16766/1/WashingtonCA.pdf https://repositorio.ufrn.br/bitstream/123456789/16766/1/WashingtonCA.pdf
institution	Acervo SISBI
collection	SIGAA
topic	Citogenética - Dissertação. Evolução cromossômica - Dissertação. Espécies crípticas - Dissertação. Peixes marinhos - Dissertação. Cytogenetic. Karyotipic evolution. Cryptic species. Marine fish.
spellingShingle	Citogenética - Dissertação. Evolução cromossômica - Dissertação. Espécies crípticas - Dissertação. Peixes marinhos - Dissertação. Cytogenetic. Karyotipic evolution. Cryptic species. Marine fish. Araújo, Washington Candeia de. Molina, Wagner Franco. Inferências carioevolutivas sobre grupos crípticos de peixes marinhos e estuarinos/
description	Resumo: The genome of all organisms is subject to injuries that can be caused by endogenous and environmental factors. If these lesions are not corrected, it can be fixed generating a mutation which can be lethal to the organisms. In order to prevent this, there are different DNA repair mechanisms. These mechanisms are well known in bacteria, yeast, human, but not in plants. Two plant models Oriza sativa and Arabidopsis thaliana had the genome sequenced and due to this some DNA repair genes have been characterized. The aim of this work is to characterized two sugarcane cDNAs that had homology to AP endonuclease: scARP1 and scARP3. In silico has been done with these two sequences and other from plants. It has been observed domain conservation on these sequences, but the cystein at 65 position that is a characteristic from the redox domain in APE1 protein was not so conservated in plants. Phylogenetic relationship showed two branches, one branch with dicots and monocots sequence and the other branch with only monocots sequences. Another approach in order to characterized these two cDNAs was to construct overexpression cassettes (sense and antisense orientation) using the 35S promoter. After that, these cassettes were transferred to the binary vector pPZP211. Furthermore, previously in the laboratory was obtained a plant from icotiana tabacum containing the overexpression cassette in anti-sense orientation. It has been observed that this plant had a slow development and problems in setting seeds. After some manual crossing, some seeds were obtained (T2) and it was analyzed the T2 segregation. The third approach used in this work was to clone the promoter region from these two cDNAs by PCR walking. The sequences obtained were analyzed using the program PLANTCARE. It was observed in these sequences some motives that may be related to oxidative stress response.#$&Abstract:Cytogenetic studies have been revealing a great diversity not detected, until then, in several families of fishes. Many of these groups, especially those that exhibit great diversity, like Perciformes and Siluriformes, possess species with difficult morphologic characterization, called cryptic species, commonly detected through karyotypic analyses, which reveals outstanding interespecific variations with relationship to the number and its chromosomal structures. Thus, the present work intends to contribute for the cytogenetic knowledge of marine and brackish fish species, because they peculiar life habits and by lack of cytogenetic data of your genetic aspects. Therefore, cytogenetic studies were developed in a species of Apogonidae (Perciformes), two species of sea catfishes of the family Ariidae (Siluriformes) and brackish fish Paurachenipterus galeatus (Siluriformes, Auchenipteridae), through C banding, Ag-NOR, use of base-specific flourochromes (DAPI and CMA3), as well as FISH (Fluorescent in situ hybridization) using ribosomal DNA probes 5S and 18S. The present results contribute to a better understanding of the processes of differentiation patterns and chromosome evolution in these groups. The use of other approaches (the morphology and molecular tools) will allow a larger understanding of the genetic and biological diversity of the Brazilian ichthyofauna.
format	Dissertação
author	Araújo, Washington Candeia de. Molina, Wagner Franco.
author_facet	Araújo, Washington Candeia de. Molina, Wagner Franco.
author_sort	Araújo, Washington Candeia de.
title	Inferências carioevolutivas sobre grupos crípticos de peixes marinhos e estuarinos/
title_short	Inferências carioevolutivas sobre grupos crípticos de peixes marinhos e estuarinos/
title_full	Inferências carioevolutivas sobre grupos crípticos de peixes marinhos e estuarinos/
title_fullStr	Inferências carioevolutivas sobre grupos crípticos de peixes marinhos e estuarinos/
title_full_unstemmed	Inferências carioevolutivas sobre grupos crípticos de peixes marinhos e estuarinos/
title_sort	inferências carioevolutivas sobre grupos crípticos de peixes marinhos e estuarinos/
publishDate	2022
url	https://repositorio.ufrn.br/bitstream/123456789/16766/1/WashingtonCA.pdf
work_keys_str_mv	AT araujowashingtoncandeiade inferenciascarioevolutivassobregruposcripticosdepeixesmarinhoseestuarinos AT molinawagnerfranco inferenciascarioevolutivassobregruposcripticosdepeixesmarinhoseestuarinos
_version_	1766816828030451712

Inferências carioevolutivas sobre grupos crípticos de peixes marinhos e estuarinos/

Registros relacionados