Invstigation of determinants of human gastric mucosa cell proliferation status using proliferating cell nuclear antigen (PCNA) immunostaining/

Disturbances in the balance between cell proliferation and loss underlie pathologic conditions of the stomach mucosa ranging from gstritis to cancer. Not much is known about factors influencing gastric mucosal cell proliferation. To investigate determinants of human gastric antrum and corpus mucosal...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Principais autores: Fernandes, Edrisi, Buset, Michel., Koster, Erik De.
Formato: Dissertação
Publicado em:
Assuntos:
Neoplasias gástricas -
Dissertação.
Células de proliferação -
Mucosa estomacal -
Infecção por helicobacter pylori -
Endereço do item:https://app.bczm.ufrn.br/home/#/item/54664
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id oai:localhost:123456789-38023
record_format dspace
spelling oai:localhost:123456789-380232022-11-29T21:00:17Z Invstigation of determinants of human gastric mucosa cell proliferation status using proliferating cell nuclear antigen (PCNA) immunostaining/ Fernandes, Edrisi Buset, Michel. Koster, Erik De. Neoplasias gástricas - Dissertação. Células de proliferação - Mucosa estomacal - Dissertação. Infecção por helicobacter pylori - Dissertação. Disturbances in the balance between cell proliferation and loss underlie pathologic conditions of the stomach mucosa ranging from gstritis to cancer. Not much is known about factors influencing gastric mucosal cell proliferation. To investigate determinants of human gastric antrum and corpus mucosal cell proliferation in vivo, looking at: A) Infection by Helicobacter pylori (HP), modality (active; chronic) and intensity of gastritis in these sites; B) Anti-HP IgG titres and intensity of gastritis of the antrum and corpus; C) HP vacuolating toxin (VTOX) status, looking also for an association between VTOX, intensity of gastritis, and mucosal cell proliferation status; and D) Sodium intake, in HP-positive (+) and negative subjetcs. We evaluated the mucosal cell proliferative status by PCNA immunostaining of antral and corpus biopsies of 81 patients (41 m, 41 f, age 18-83 ys). Proliferation status was estimated by the PCNA labelling index (LI). Histology and HP culture of antrum and corpus were performed according to the Sidney System. Serology was performed by ELISA. The presence of VTOX was determinated in a cell vacuolation assay. Sodium (Na) intake was stimated by the Na/creatinine ratio in fresh urine samples. 1) HP was detected in 50 individuals. A correlation (C), significant, was found between presence of HP and antrum PCNA LI (Mann-Whitney, p=0,0073). A weak association between intensity of inflammation and PCNA LI was found for active and chronic gastritis (CG) of the antrum and for CG of the corpus (Spearman's r=0,37, 0,46, 0,28, respectively); 2) A C was found between serotitres and active and CG in the antrum, and CG in the corpus (r=0,59, 0,64, 0,58, respect). A C was also found between serotitres and antrum PCNA LI (r=0,42; in HP (+) patients, r=0,46). 3) A weak C was detected between VTOX and corpus PCNA LI (p=0,02); 4) Sodium intake correlated, but not strongly with#$&antrum PCNA LI (r=0,31). Conclusion: 1) HP infection, presence of gastric mucosal inflammation, serology, presence of HP VTOX activity and Na intake correlate individually, but not strogly, with antrum and corpus mucosal cell proliferative status in humans; 2) Antrum and corpus proliferation status are influenced by different determinants. 1 2022-10-05T11:57:07Z 2022-10-05T11:57:07Z 1994. Dissertação 616.33-006 F363i DISSERT 54664 https://app.bczm.ufrn.br/home/#/item/54664 https://app.bczm.ufrn.br/home/#/item/54664
institution Acervo SISBI
collection SIGAA
topic Neoplasias gástricas -
Dissertação.
Células de proliferação -
Mucosa estomacal -
Dissertação.
Infecção por helicobacter pylori -
Dissertação.
spellingShingle Neoplasias gástricas -
Dissertação.
Células de proliferação -
Mucosa estomacal -
Dissertação.
Infecção por helicobacter pylori -
Dissertação.
Fernandes, Edrisi
Buset, Michel.
Koster, Erik De.
Invstigation of determinants of human gastric mucosa cell proliferation status using proliferating cell nuclear antigen (PCNA) immunostaining/
description Disturbances in the balance between cell proliferation and loss underlie pathologic conditions of the stomach mucosa ranging from gstritis to cancer. Not much is known about factors influencing gastric mucosal cell proliferation. To investigate determinants of human gastric antrum and corpus mucosal cell proliferation in vivo, looking at: A) Infection by Helicobacter pylori (HP), modality (active; chronic) and intensity of gastritis in these sites; B) Anti-HP IgG titres and intensity of gastritis of the antrum and corpus; C) HP vacuolating toxin (VTOX) status, looking also for an association between VTOX, intensity of gastritis, and mucosal cell proliferation status; and D) Sodium intake, in HP-positive (+) and negative subjetcs. We evaluated the mucosal cell proliferative status by PCNA immunostaining of antral and corpus biopsies of 81 patients (41 m, 41 f, age 18-83 ys). Proliferation status was estimated by the PCNA labelling index (LI). Histology and HP culture of antrum and corpus were performed according to the Sidney System. Serology was performed by ELISA. The presence of VTOX was determinated in a cell vacuolation assay. Sodium (Na) intake was stimated by the Na/creatinine ratio in fresh urine samples. 1) HP was detected in 50 individuals. A correlation (C), significant, was found between presence of HP and antrum PCNA LI (Mann-Whitney, p=0,0073). A weak association between intensity of inflammation and PCNA LI was found for active and chronic gastritis (CG) of the antrum and for CG of the corpus (Spearman's r=0,37, 0,46, 0,28, respectively); 2) A C was found between serotitres and active and CG in the antrum, and CG in the corpus (r=0,59, 0,64, 0,58, respect). A C was also found between serotitres and antrum PCNA LI (r=0,42; in HP (+) patients, r=0,46). 3) A weak C was detected between VTOX and corpus PCNA LI (p=0,02); 4) Sodium intake correlated, but not strongly with#$&antrum PCNA LI (r=0,31). Conclusion: 1) HP infection, presence of gastric mucosal inflammation, serology, presence of HP VTOX activity and Na intake correlate individually, but not strogly, with antrum and corpus mucosal cell proliferative status in humans; 2) Antrum and corpus proliferation status are influenced by different determinants.
format Dissertação
author Fernandes, Edrisi
Buset, Michel.
Koster, Erik De.
author_facet Fernandes, Edrisi
Buset, Michel.
Koster, Erik De.
author_sort Fernandes, Edrisi
title Invstigation of determinants of human gastric mucosa cell proliferation status using proliferating cell nuclear antigen (PCNA) immunostaining/
title_short Invstigation of determinants of human gastric mucosa cell proliferation status using proliferating cell nuclear antigen (PCNA) immunostaining/
title_full Invstigation of determinants of human gastric mucosa cell proliferation status using proliferating cell nuclear antigen (PCNA) immunostaining/
title_fullStr Invstigation of determinants of human gastric mucosa cell proliferation status using proliferating cell nuclear antigen (PCNA) immunostaining/
title_full_unstemmed Invstigation of determinants of human gastric mucosa cell proliferation status using proliferating cell nuclear antigen (PCNA) immunostaining/
title_sort invstigation of determinants of human gastric mucosa cell proliferation status using proliferating cell nuclear antigen (pcna) immunostaining/
publishDate 2022
url https://app.bczm.ufrn.br/home/#/item/54664
work_keys_str_mv AT fernandesedrisi invstigationofdeterminantsofhumangastricmucosacellproliferationstatususingproliferatingcellnuclearantigenpcnaimmunostaining
AT busetmichel invstigationofdeterminantsofhumangastricmucosacellproliferationstatususingproliferatingcellnuclearantigenpcnaimmunostaining
AT kostererikde invstigationofdeterminantsofhumangastricmucosacellproliferationstatususingproliferatingcellnuclearantigenpcnaimmunostaining
_version_ 1766861429071151104

Registros relacionados